購買熒光標(biāo)記的Annexin V，可獲得京東購物卡一張！

Cell Meter  PE-Annexin V細(xì)胞凋亡檢測試劑盒是美國AAT Bioquest生產(chǎn)的用于檢測細(xì)胞凋亡的試劑盒，膜聯(lián)蛋白V可以與包括PE的熒光染料結(jié)合。該染料保留了其對磷脂酰絲氨酸（PS）的高親和力，因此可用作流式細(xì)胞術(shù)分析正在經(jīng)歷細(xì)胞凋亡的細(xì)胞的敏感探針。由于PS的外化發(fā)生在細(xì)胞凋亡的早期階段，PE膜聯(lián)蛋白V染色可以比基于核變化（例如DNA片段化）的測定更早地鑒定細(xì)胞凋亡。

數(shù)據(jù)分析

在活的非凋亡細(xì)胞中，RPE-膜聯(lián)蛋白V檢測非誘導(dǎo)細(xì)胞中的先天性細(xì)胞凋亡，其通常為所有細(xì)胞的2-6％。 在凋亡細(xì)胞中，RPE-膜聯(lián)蛋白V與磷脂酰絲氨酸結(jié)合，磷脂酰絲氨酸位于細(xì)胞膜的外葉上，因此導(dǎo)致染色強(qiáng)度增加。

參考文獻(xiàn)

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Detection of apoptosis based on the interaction between annexin V and phosphatidylserine
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Journal: Anal Chem (2009): 2410

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evaluation of cell surface expression of phosphatidylserine in ovarian carcinoma effusions using the annexin-V/7-AAD assay: clinical relevance and comparison with other apoptosis parameters
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